BIOL 4513 - Molecular Biology of Nucleic Acids with Laboratory

Prerequisites: undergraduate biochemistry, and molecular biology or genetics, or permission of the instructor. This course uses an intensive laboratory component to instruct students in the practical and technical aspects of working with nucleic acids. Provides an examination of the techniques available for manipulating DNA and RNA including nucleic acid extraction and quantitation, recombinant DNA techniques, PCR, DNA sequencing and mutation analysis, and techniques for examining gene expression including expression cloning, reverse transcriptase PCR and quantitative real time PCR. The ability to effectively communicate laboratory findings is emphasized through frequent writing assignments. Laboratory fee. 4 credits