CH 640 - Chemical Separations

Prerequisites: Evidence of mastery of the concepts of chemistry as demonstrated with a BS degree in chemistry or biology. Students should have courses equivalent to UNH courses CH 202 Organic Chemistry and CH 221 Instrumental Methods of Analysis. Biological systems contain many thousands of different organic compounds that are present at very low concentrations. This course deals with current methods of separating, detecting, and quantifying pharmaceuticals and associated metabolites and other "small molecule" organic agents present in complex animal and agricultural samples. Clean-up methods include liquid and solid phase extractions, gel filtration, size-exclusion, ion-exchange, and affinity chromatography. Analytical methods emphasize HPLC, GC with MS and flourescence detection, and detection-oriented derivatization. Comparison and evaluation of different techniques are presented with practical examples.
3 credits