CMBI 6611 - Molecular Biology of Proteins with Laboratory

Prerequisites: undergraduate biochemistry, molecular biology or genetics or consent of the instructor. This course uses an intensive laboratory component to instruct students in molecular biology techniques for studying protein characteristics and extend beyond the understanding of basic protein biochemistry. Students learn: how physical properties of proteins impact our ability to work with proteins in the laboratory; how to purify protein using chromatographic techniques; how to express and purify proteins for enzymatic functional studies; and how to purify proteins from various cells or tissues and examine their expression and activity by two-dimensional polyacrylamide gel electrophoresis. The ability to effectively communicate laboratory findings is emphasized through frequent writing assignments. Laboratory fee. 4 credits.